Which tests are needed for further identification of a gram-positive cocci that is catalase negative and B-hemolytic in a pregnant woman?

The identification of a catalase-negative, β-hemolytic gram-positive cocci in a pregnant woman is crucial, particularly in distinguishing between Streptococcus species, such as Streptococcus pyogenes and Streptococcus agalactiae (Group B Streptococcus, GBS), both of which can have significant implications during pregnancy.

The tests listed are designed to determine the specific characteristics of the organism. Bacitracin sensitivity is key in identifying Streptococcus pyogenes, as this organism is sensitive to bacitracin, whereas Streptococcus agalactiae is resistant. The CAMP test is another important diagnostic test that reveals the presence of Streptococcus agalactiae; it can be identified by its synergy in producing enhanced hemolysis in the presence of Staphylococcus aureus. PYR (pyrrolidonyl arylamidase) testing is used to differentiate between S. pyogenes, which tests positive for PYR, and S. agalactiae, which tests negative.

Together, these tests accurately confirm the identity of either S. pyogenes or S. agalactiae. This information is particularly relevant to pregnant women due to the potential risks associated with Group B Strept