Which reagent is used to detect the presence of indole?

Get ready for the Harr Microbiology Test. Leverage flashcards, multiple choice questions with hints and explanations. Excel in your exam preparation!

The presence of indole is detected using p-Dimethylaminobenzaldehyde, commonly known as Kovac's reagent. When this reagent is added to a culture medium that contains tryptophan, it reacts with indole, which is produced when tryptophan is broken down by certain bacteria. The reaction typically results in the formation of a red or pink ring at the top of the medium, indicating a positive test for the presence of indole.

The other reagents listed do not specifically react with indole. Bromcresol purple is used as a pH indicator and is not involved in indole detection. Methyl red serves as an indicator for mixed acid fermentation but does not interact with indole. Cytochrome oxidase is a reagent used to test for the presence of cytochrome c oxidase in certain bacteria, unrelated to indole detection. Thus, p-Dimethylaminobenzaldehyde is the appropriate choice for identifying indole production in microbial culture.

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