Which procedure is best for detecting motility in organisms?

The procedure that is best for detecting motility in organisms is the direct wet examination. This method allows for the observation of live organisms under a microscope in a droplet of liquid. Since motile bacteria can swim and move in this environment, they can be easily visualized as they move across the field of view. This real-time observation makes it the most effective technique for assessing motility compared to other methods.

In contrast, while stool culture can provide information about the presence of specific bacteria, it does not allow for direct observation of motility since organisms may be inactive after being cultured. Histological staining techniques are primarily used to visualize morphology and tissue structures rather than assess motility. Lastly, induced sputum examination is more useful in identifying pathogens in respiratory specimens but does not focus on evaluating motility, as it is primarily aimed at diagnostic purposes rather than observing live motility.