What is the correct sequence for skin cleansing during the aseptic collection of blood cultures?

The correct sequence for skin cleansing during the aseptic collection of blood cultures is to first use a 70% alcohol solution followed by 2% iodine or an iodophor. This method is effective because 70% alcohol serves as a rapid antiseptic that helps to denature proteins and kill many types of bacteria effectively. By applying it first, it reduces the microbial load on the skin quickly.

Following the application of alcohol, the 2% iodine or iodophor is used to further disinfect the area. Iodine has a broader spectrum of antimicrobial activity and, when used after alcohol, provides a thorough and prolonged antiseptic effect. This combination ensures that any remaining microbes present after the initial alcohol application are effectively eliminated before the blood culture is obtained.

Using this sequence maximizes the efficacy of antimicrobial activity by leveraging the properties of both agents, which together create a sterile environment ideal for blood culture collection. Other listed options may not utilize the effective synergy between the alcohol and iodine or do not comply with best practices in infection control.