What concentration of sodium hydroxide (NaOH) is used to prepare a working decontamination solution for mycobacteria?

The preparation of a working decontamination solution for mycobacteria typically involves using a concentration of 4% sodium hydroxide (NaOH). This concentration is effective for the decontamination of mycobacterial specimens, as it provides a sufficient strength to disrupt and inactivate mycobacteria while being manageable for laboratory use. Mycobacteria, such as Mycobacterium tuberculosis, are known for their resilience and thick, waxy cell walls, which require a more potent decontamination solution to eliminate their presence effectively in clinical samples.

Higher concentrations of NaOH may be damaging to the sample or too caustic for handling, while lower concentrations may not offer adequate decontamination. Consequently, 4% NaOH strikes a balance, allowing for effective mycobacterial disinfection without compromising sample integrity or safety during application. Understanding this standard is essential when preparing samples for mycobacterial culture and ensuring lab safety protocols are met.