Identify the incorrect match between method and its objective.

The identification of the incorrect match focuses on understanding the specific objectives associated with each method of examination in microbiology.

In the case of Knott's concentration, this method is primarily used for recovering microfilariae from blood samples, especially for detecting filarial parasites. Although it concentrates parasites and can assist in identifying various types of eggs, it is not typically employed for the specific recovery of operculated helminth eggs. Operculated eggs are usually associated with certain types of trematodes and are better identified through other methods specifically designed for examining fecal samples rather than blood.

On the other hand, the direct wet examination is a suitable technique for assessing organism motility, as it allows visualization of living organisms in a sample. Baermann's concentration method is accurately matched with its objective of recovering Strongyloides larvae from stool specimens or tissue, as it utilizes a setup that encourages the larvae to move into a collection apparatus. Finally, the permanent stained fecal smear is appropriately used for confirming the presence of protozoa, as staining enhances visualization and differentiation of protozoan cysts and trophozoites.

Understanding these specific techniques and their primary applications is crucial for microbiological diagnostics, highlighting the inaccuracy of considering Knott's concentration as