Haemophilus influenzae, which causes ocular infections, requires which factors in the primary medium for growth?

Haemophilus influenzae is a fastidious organism that necessitates specific growth factors for successful cultivation. In a laboratory setting, this bacterium requires both factor X (hemin) and factor V (nicotinamide adenine dinucleotide, or NAD) to grow.

Factor X, which is essential as it provides heme, is critical for various cellular processes, including the synthesis of enzymes necessary for metabolism. Factor V acts as a coenzyme for key metabolic reactions. The absence of these factors in a primary medium will inhibit the growth of Haemophilus influenzae. Therefore, when provided with a medium supplemented with both X and V factors, the bacterium can thrive and be studied, which is why this answer is correct.

Other choices do not refer to known growth factors relevant to Haemophilus influenzae, and therefore do not provide the necessary conditions needed for the growth of this organism in laboratory cultures.