At what temperature does Mycobacterium marinum optimally grow on Lowenstein-Jensen agar?

Mycobacterium marinum is known to thrive at a temperature of approximately 30°C, which correlates with its natural habitat. This species is typically associated with aquatic environments, making the slightly lower temperature optimal for its growth compared to other Mycobacterium species that prefer higher temperatures, such as Mycobacterium tuberculosis, which optimally grows around 37°C. The use of Lowenstein-Jensen agar, which is specifically formulated for the isolation of mycobacteria, supports aerobic growth and provides the necessary nutrients for Mycobacterium marinum under these optimal conditions. Understanding the temperature requirements of different mycobacteria is crucial for accurate laboratory identification and for ensuring successful culture conditions.